Preparation of library DNA is an important process required for generating valuable Next Generation Sequencing data. Enzymatics has developed lyophilized room temperature stable library construction kits for the Illumina® and Ion Torrent™ sequencing platforms. Each SPARK™ DNA Sample Prep kit provides lyophilized room temperature stable reaction master mixes for the preparation of adapter ligated DNA libraries for Next Generation Sequencing from sheared double-stranded DNA. The kits are designed for constructing genomic DNA libraries with 10 ng to 1000 ng of input DNA and provide:

  • Simplified workflow
  • Reduced pipetting
  • Consistent yield

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Reduce GC-bias and time spent in NGS library preparation with SPARK™ DNA Sample Prep Kits

Sequencing Library Research at the Sanger Institute

Michael Quail, Ph.D. — Sequencing Research Team Leader at the Sanger Institute highlights using the SPARK™ DNA Sample Prep Kits to reduce GC-bias in constructing next-gen sequencing libraries and performing targeted enrichment of AML genes using the Archer AMP technology

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Low Input FFPE

Formalin-Fixed Paraffin-Embedded (FFPE) samples are often challenging for molecular assays, such as Next Generation Sequencing (NGS), due to nucleic acid damage and base modification. The SPARK™ DNA Sample Prep Kit, along with 2X VeraSeq™ PCR Mix, was designed to handle these difficult samples.

Workflow

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Applications

  • De Novo
  • Re-Sequencing
  • Low Input FFPE