| Product Information |
| Mako DNA Polymerase (3′→ 5′ exo-) |
| Part Number: |
P709L |
| Price: |
$250 |
Product Description:
Mako DNA Polymerase (3′→ 5′ exo-) catalyzes the extension of a primed DNA template in the 5′→3′ direction. This enzyme lacks any inherent 3′→ 5′ and 5′→ 3′ exonuclease activities and exhibits no measurable strand displacement function.
Source of Protein
Purified from a strain of E. coli that expresses the recombinant Mako DNA Polymerase (3′→ 5′ exo-) gene.
Supplied in:
100 mM KPO4
1.0 mM dithiothreitol
0.1 mM EDTA
50% Glycerol
pH 6.5 @ 25°C
Supplied With:
B011 (10X Blue Buffer)
10X Blue Buffer (B011)
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
Nuclease Contamination Tests:
Single-Stranded Exonuclease Activity
A 50 µL reaction containing 11,000 cpm of a radiolabeled single-stranded DNA substrate and 10 µL of enzyme solution incubated for 4 hours at 37°C resulted in less than 5.0% release of TCA-soluble counts.
Double-Stranded Exonuclease Activity
A 50 µL reaction containing 5,000 cpm of a radiolabeled double-stranded DNA substrate and 10 µL of enzyme solution incubated for 4 hours at 37°C resulted in less than 0.5% release of TCA-soluble counts.
Endonuclease Activity
A 50 µL reaction containing 0.5 µg of pBR322 DNA and
10 µL of enzyme solution incubated for 4 hours at 37°C resulted in no visually discernible conversion to nicked circular DNA as determined by agarose gel electrophoresis.
E.coli 16S rDNA Contamination Test
Replicate 5 µL samples of enzyme solution were heat denatured and screened in a TaqMan qPCR assay for the presence of contaminating E.coli genomic DNA using primers for the 16S rRNA locus. The acceptance criterion for the test is the threshold cycle count (Ct) produced by the average of 3 replicate no template control samples. Based on no template control Ct values, the detection limit of this assay is <10 copies genome/sample.
|
| Product Specification* |
| Unit Size: |
3,000 Units |
| Unit Concentration |
30,000 U/mL |
| Protein Concentration |
4.92 mg/mL |
| Purity (SDS-PAGE) |
>99% |
| Specific Activity |
6,100 U/mg |
| SS Exonuclease |
300 U <5.0% released |
| DS Exonuclease |
300 U <0.5% released |
| Endonuclease |
300 U <10% converted |
| E.coli 16S rDNA Contamination |
300 U <10 copies |
| Storage |
-20°C |
* For a detailed summary of assay conditions and data, refer to the
Quality Controls Analysis section below
Quality Control Analysis:
Unit Characterization Assay
Unit activity was measured using a 2-fold serial dilution method. Dilutions of enzyme were made in 1X reaction buffer ([Mako]f = 0.25-0.002µg/µL) and added to 50 µL reactions containing 10µg denatured Calf Thymus DNA, 1X Blue Reaction Buffer, 4mCi/mL 3H-dTTP and 100 µM dNTPs. Reactions were incubated 10 minutes at 37°C, plunged on ice, and analyzed using the method of Sambrook and Russell (Molecular Cloning, v3, 2001, pp. A8.25-A8.26).
Protein Concentration (OD280) Measurement
A 2.0 µL sample of enzyme was analyzed at OD280 using a Nanodrop ND-1000 spectrophotometer standardized using a 2.0 mg/ml BSA sample (Pierce Cat #23209) and blanked with product storage solution. The observed average measurement of 3 replicate samples was converted to mg/mL using an extinction coefficient of 128,440 and molecular weight of 103,565 Daltons. Acceptance for this assay is +/- 5% of reference sample.
SDS-Page (Physical Purity Assessment)
2.0 µL of enzyme solution was loaded on a denaturing 4-20% Tris-Glycine SDS-PAGE gel flanked by a broad-range MW marker and 2.0 µL of a 1:100 dilution of the sample. Following electrophoresis, the gel was stained and the samples compared to determine physical purity. The acceptance criteria for this test requires that the aggregate mass of contaminant bands in the concentrated sample do not exceed the mass of the protein of interest band in the dilute sample, confirming greater than 99% purity of the concentrated sample. |
