VeraSeq PCR Mix is a premixed, ready-to-use 2X solution containing VeraSeq 2.0 High-Fidelity DNA Polymerase, dNTPS, MgCl2 and reaction buffer at optimal concentrations to maximize the speed, accuracy, and length of DNA synthesis. It is formulated to provide efficient, high-fidelity DNA amplification for Next Generation Sequencing library preparation, cloning, and synthetic biology applications.
Functionality of 2X VeraSeq PCR Mix is assessed by its ability to amplify a 500bp fragment from genomic DNA. Following PCR the 500bp fragment was visualized by Agarose gel electrophoresis.
VeraSeq was tested prior to assembly and found free of contaminating endonucleases. Enzyme purity was >99% as determined by SDS-PAGE and negligible E.coli genomic DNA contamination was confirmed by qPCR. Specific activity was verified pre and post dilution.
|NGS Library Prep: 2X VeraSeq™ PCR Mix – 250 Reactions|
|Unit Size||250 Reactions|
|SDS||Available on request|
|Storage Temperature||-25°C to -15°C|
|Functional Assay||Amplification of 500bp fragment from Genomic DNA|
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
Ideal choice for applications requiring high fidelity DNA amplification such as cloning, next generation sequencing, and synthetic biology.
VeraSeq 2.0 is licensed from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,560,260 and corresponding patents in other countries for use solely in DNA sequencing, DNA micro-array, and conventional PCR applications, including pre-amplification steps that are required for such applications, in the life science research fields but not real-time PCR or digital PCR. In-vitro diagnostics rights are available, please contact Enzymatics for more information.
Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for administration to humans or animals. MSDS sheets relevant to this product are available upon request.
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