The ZipScript One-Step RT-qPCR Kit is a highly sensitive and reproducible RT-qPCR solution optimized for real-time PCR. The 25X enzyme mix is accompanied by a 2X reaction buffer. If a passive reference dye, such as ROX, is required for your assay, it can be purchased separately (P/N: Y9390).
The functionality of the ZipScript is evaluated by amplification of two mRNA transcripts in a one-step quantitative RT-qPCR assay. The amplification threshold (Ct) of the test lot is compared to a reference lot.
25X ZipScript Enzyme Mix 2X ZipScript Reaction Buffer I
Enzyme components were tested prior to formulation of the master mix and found free of contaminating endonucleases and exonucleases. Enzyme purity was >99% as determined by SDS-PAGE and negligible E.coli genomic DNA contamination was confirmed by qPCR. Specific activity was verified for each enzyme pre-formulation.
ZipScript One-Step RT-qPCR Reaction Setup:
|Nuclease-Free Water||To a final 20 µL reaction volume||–|
|2X ZipScript Reaction Buffer I||10 µL||11X|
|50X ROX (optional)||0.4 µL||1X|
|Primer/Probe Mix||X µL||Variable|
|25X ZipScript Enzyme Mix||0.8 µL||0.8 µL|
Thermal Cycling Conditions:
Program the cycling conditions based on the recommendations below.
Standard Cycling Program*
|Reverse Transcription||50°C||15 min||1|
|Taq Activation/InitialDenaturation||95°C||2 min||1|
* Cycling parameters can be modified (especially the annealing/extension condition) to fit specific primer/probe selection.
|ZipScript™ One-Step RT-qPCR Kit|
|Unit Size||1000 Reactions|
|SDS||Available on request|
|Storage Temperature||-25°C to -15°C|
|Specification||Amplification of Test Lot within 1Ct of Reference Lot for two different mRNA targets|
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
Limitations of Use
This product was developed, manufactured, and sold for in vitro use only. The product is not suitable for administration to humans or animals. SDS sheets relevant to this product are available upon request.
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